This page was last modified on Sunday, May 12, 2013 03:21:41 PM
Welcome to the
Case Western Reserve University School of Medicine
Alex Y. Huang, MD, PhD
Case Western Reserve University /
University Hospitals Case Medical Center /
Rainbow Babies & Children's Hospital
Cleveland, Ohio USA
Upcoming Seminar Engagement...
- American Society of Colon-Rectal Surgeons Annual Meeting, April 29th (Bobanga)
- Cancer Research Institute Annual Meeting, September 30th - October 2nd (Huang)
Huang Lab Photos
Focus of our laboratory:
Regulation between immune activation and tolerance is crucial in the pathogenesis of cancer. Recent development of intravital 2-photon microscopy allows direct observation of how immune cells traffic and interact with various cells in the tumor micro-environment in real time with single-cell resolution.
Our laboratory is interested in applying this and other classical immunological techniques to study various aspects of anti-tumor immune responses, immune – host – pathogen interaction, T cell-mediated memory immunity, and chemokine - receptor biology. Ongoing projects include:
1) Investigation into factors which influence the dynamic recruitment of T cells and their interactions with tumor cells and associated antigen-presenting cells in tumor-associated micro-environment
2) Imaging dynamic interaction between peri-vascular APC and Th17 cells in the CNS during early induction of acute experimental autoimmune encephalomyelitis
3) Discovering molecular signaling mechanisms responsible for the regulation of chemokine receptor expression on naive T cells
4) Testing the efficacy of genetically-modified tumor vaccines in the setting of sarcomas and other childhood cancer
5) Developing imaging techniques to interrogate immune cell migration and interactions in tissues such as the bone marrow, lung, kidney, GI track and the skin.
The long-term goal of our laboratory is to translate insights gained from basic investigations of in vivo immunity into rationally designed tumor vaccine and immunotherapeutic clinical trials, with a particular emphasis in the areas of pediatric sarcomas and other solid tumors.
Barkauskas DS*, Evans TA*, Myers J, Petrosiute A, Silver J, Huang AY. Extravascular CX3CR1+ Cells Extend Intravascular Dendritic Processes into Intact Central Nervous System Vessel Lumen. Microscopy & Microanalysis 2013; May 3:1-13 [Epub ahead of print].
Myers J, Barkauskas DS, Huang AY. Dynamic imaging of marrow-resident granulocytes interacting with human mesenchymal stem cell upon systemic lipopolysaccharide challenge. Stem Cell International. 2013; 2013:656839.
Huang AY, Haining WN, Barkauskas DS, Myers J, Petrosiute A, Garrett AP, Singh K, Cooke K, Kean LS. Viewing transplantation immunology through today's lens: new models, new imaging, and new insights. Biol. Blood Marrow Transplant. 2013; 19(1 Suppl):S44-51
Mandl JN, Liou R, Klauschen F, Vrisekoop N, Monteiro JP, Yates AJ, Huang AY*, Germain RN*. Quantification of lymph node transit times reveals differences in antigen surveillance strategies of naive CD4+ and CD8+ T cells. Proc. Natl. Acad. Sci. USA 2012; doi/10.1073/pnas.1211717109 (*co-senior author)
Huang AY, Myers JT, Barkauskas D, Howell SJ, Oleinick NL, McCormick TS, Cooper KD, Baron ED, Lam M. Cutaneous penetration of the topically applied photosensitizer Pc4 as detected by intravital 2-photon laser scanning microscopy. Phtodiag. Photodyn. Ther. 2012; 9*3):225-231.
Liou HLR, Myers JT, Barkauskas DS, Huang AY. Intravital imaging of the mouse popliteal lymph node. J. Vis. Exp. 2012; Feb 8 (60); pii: 3720. doi:10.3791/3720
Lathia JD, Gallagher J, Myers JT, Li M, Vasanji A, McLendon RE, Hjelmeland AB, Huang AY, Rich JN. In vivo evidence for tumor propagation by glioblastoma cancer stem cells. PLoS One. 2011; 6(9):e24807. Epub 2011 Sep 22.
Pareek, TK, Lam E, Zheng X, Askew D, Kulkarni AB, Chance MR, Huang AY, Cooke KR, Letterio JJ. Cyclin-dependent kinase 5 activity is required for T cell activation and induciton of experimental autoimmune encephalomyelitis. J. Exp. Med. 2010; 207(11):2507-2519.
Simmons DP, Canaday DH, Liu Y, Li Q, Huang A, Boom WH, Harding CV. Mycobacgterium tuberculosis and TLR2 agonists inhibit induction of type I IFN and class I MHC antigen cross processing by TLR9. J. Immunol. 2010; 185(4):2405-2415.
Myers J, Huang Y, Wei L, Yan Q, Huang A, Zhou L. Fucose-deficient hematopoietic stem cells have decreases self-renewal and aberrant marrow niche occupancy. Transfusion 2010; 50(12):2660-2669.
Huang AY. Bulging glands? Blame it on B cells. Blood. 2010; 115(23):4624- 4626.
Germain RN, Bajenoff M, Castellino F, Chieppa M, Egen JG, Huang AYC, Ishii M, Koo LY, Qi H. Making friends in out-of-the-way places: how cells of the immune system get together and how they conduct their business as revealed by intravital imaging. Immunological Reviews 2008; 221:163-181
Bajenoff M, Egen JG, Qi H, Huang AY, Castellino F, Germain RN. Highways, byways and breadcrumbs: directing lymphocyte traffic in the lymph node. Trends Immunol. 2007, 28(8): 346-352.
Guarda G, Hons M, Soriano SF, Huang AY, Polley R, Martin-Fontecha A, Stein JV, Germain RN, Lanzavecchia A, Sallusto F. L-selectin- CCR7- effector and memory CD8+ T cells enter reactive lymph nodes and kill dendritic cells. Nat. Immunol. 2007, 8(7):743-52.
Huang AY. Watching Immune Cells in Action. Biology of Blood and Marrow Transplantation. 2007, 13:111-114.
Chieppa M, Rescigno M, Huang AY, Germain RN. Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TCL engagement. J Exp Med. 2006, 203:2841-52.
Qi H, Egen JG, Huang AY, Germain RN. Extrafollicular activation of lymph node B cells by antigen-bearing dendritic cells. Science 2006, 312-1672-76.
Castellino F*, Huang AY*, Altan-Bonnet G, Stoll S, Scheinecker C, Germain RN. Chemokines enhance immunity by guiding naive CD8+ T cells to sites of CD4+ T cell-dendritic cell interaction. Nature 2006, 440:890-95 (*shared first authorship).
Bajenoff M, Breart B, Huang AY, Qi H, Cazareth J, Braud VM, Germain RN, Glaichenhaus N. Natureal killer cell behavior in lymph nodes revealed by static and real-time imaing. J Exp Med. 2006, 203:619-31.
Germain RN, Castellino F, Chieppa M, Egen JG, Huang AY, Koo LY, Qi H. An extended vision for dynamic high-resolution intravita immune imaging. Semin Immunol. 2005, 17:431-41.
Huang AY, Qi H, Germain RN. Illuminating the landscape of in vivo immunity: insights from dynamic in situ imaging of secondary lymphoid tissues. Immunity 2004, 21:331-339.
[for a complete list of publications, please see ABOUT US page]
Huang Lab NEWS
Congratulations to Iuliana Bobanga, MD for winning first place at the annual meeting of the Ohio Chapter of American Surgical Society on May 3rd.
May, 2013We are grateful to receive the support and partnership of The Littlest Heroes Vs. Cancer Foundation. Please check out their website and give them your full support!
The Huang lab has just received a 2-year, $250,000 grant from Hyundai "Hope-on-Wheels" Hope Award to study the role of Cdk's in ICN1-induced T-ALL. Hand print ceremony will occur in the Fall at the University Hospitals.
More details to come. Stay tuned.
Congratulations to Fred Allen for being awarded a Minority Scientist Travel Award at the upcoming American Association of Immunologists (AAI) Annual Meeting in Hawaii
March 31, 2013
Huang Lab and Team Rainbow Head Shaving Fundraising Effort on behalf of St. Baldrick's Foundation was featured on the Steven G. AYA Cancer Research Fund website.
March 18, 2013
A special ceremony took place in the Rainbow Performance Area on Monday morning as local event organizers, volunteers and shavees of the 2013 St. Baldrick's Foundation Fundraising Event Cleveland Chapter came together to celebrate and welcome Kathleen Ruddy, CEO of St. Baldrick's Foundation. Watch and read about this event as reported on Channel 5 News.
March 14, 2013
Member of the Huang Lab had another great showing this year at the annual St. Baldrick's Foundation Head Shaving Event at Rainbow Babies & Children's Hospital. Deb Barkauskas has de-throned Alex this year as the top fundraiser on Team Rainbow, and more money is pouring in. Please consider contributing to this most worthy cause in fueling research funding for Childhood Cancer Research. See Fox8 New coverage of this event.
February 26, 2013
Huang lab members have started the annual ritual of getting heads shaved to raise funds in support of St. Baldrick's Foundation, a terrific foundation which supports Pediatric Hematology-Oncology Research. Please check out Team Rainbow and support this worthy cause.
The Head-Shaving Date
is Thursday, March 14th from 10:30am to 12:30pm in the Performance Area of Rainbow Babies & Children's Hospital.
January 26, 2013
Huang lab members were honored at the annual Steven G. AYA Research Fund fundraiser dinner. Drs. David Askew, Iuliana Bobanga, Agne Petrosiute, Alex Huang and Mrs. Deborah Barkauskas were in attendance to help support this worthy cause.
November 13, 2012
Dr. Huang spoke at the annual meeting of the Gabrielle's Angel Foundation in New York's Central Park Loeb Boathouse. Check out the video where Dr. Huang (0:52) and other speakers at the symposium spoke of the importance of the funding from GAF in supporting many of our ongoing research initiatives.
November 1, 2012Congratulations to Dr. David Askew who was named the 2012 Recipient of the Steven G. AYA Cancer Research Scholarship. The entire Huang Lab is grateful for the support and partnership of the Steven G. AYA Cancer Research Fund! Read more about Dr. Askew's Award and please be sure to support the Steven G. AYA Cancer Researh Fund.
Thank you, Steven, Angie and Harry!
Congratulations to Dixon Dorand for being named recipient of the CWRU ITP T32 Training Grant Recipient for his study into antigen processing and presentation pathways of CNS Tumor-associated antigens. Congratulations Dixon.
September 1, 2012
Congratulations to Fred Allen for receiving a two-year NIH NCI Diversity Supplement Grant to study intranodal immune cell migration in metastatic colon tumors. Congratulations, Fred!
September 12, 2012
Congratulations to Dr. Agne Petrosiute, who received the 2012 Hyundai Hope-on-Wheel Program Research Award in the amount of $75,000. Congratulations, Agne!
Watch local Cleveland Fox8 News and 19 Action News Coverage of the event and Agne's speech...
July 6, 2012
Read the latest press release regarding ALSF Innovator Award in Case Daily
We are fortunate to be visited by Harry and Angie Giallourakis from the Steven G AYA Cancer Research Fund on May 18th. We are so grateful for their passion and dedication to finding a cure for AYA cancer. Read the blog about the Huang Lab on their website.
Dr. Francesca Scrimieri just received appointment to the Orthopedic Department's T32 Training Program for her ongoing work in osteosarcoma.
Member of the Huang Lab participated in the St. Baldrick's Foundation's annual head-shaving fundraising event as part of the Rainbow Babies & Children's Team. Team Rainbow had over 33 shaves, raising more than $27,000 (second top team in Cleveland). Dr. Huang raised $4900, placing him on top-ten list of individual fund-raisers from the City of Cleveland. To celebrate Cleveland's 10th anniversary in participating in this most worthy cause, Team Rainbow hosted its first-ever hair-shaving event in the Atrium of University Hospitals on March 9th.
Deborah Barkauskas received appointment to the Biomedical Engineering Department's T32 Training Program for her ongoing work in CNS imaging.
Congratulations to Deborah Barkauskas for winning best poster presentation at the 2011 Annual International Microscopy & Microanalysis Meeting in Nashville, TN.
Congratulations to Dr. Agne Petrosiute, MD for being awarded the FRAP Career Development Award. This $40,000 award is given to one senior fellow-junior faculty each year in the Department of Pediatrics in support and recognition of his/her accomplishment in research.
Congratulations to Youmna Othman, MD for being chosen as a recipient of the St. Baldrick's Fellow Scholarship through a competitive national grant review process for her proposal to monitor T cell ALL infiltration in the CNS through the Blood-Brain Barrier in a spontaneous murine leukemia model. This award is for a 2-year period in the amount exceeding $155,000.
Dr. Alex Huang has received an additional $230,000 grant from the St. Baldrick's Foundation for the laboratory's effort to define immune surveillance in pediatric sarcomas and other cancers.
Congratulations to Deborah Barkauskas for being named the 2011 Distinguished Scholar Award in the upcoming Microscopy & Microanalysis 2011 meeting in Nashville, TN for her paper entitled, Monitoring Immune Cell Infiltration in the Brain with Intravital Two-Photon Microscopy. She will receive compensation to attend the annual meeting in August, and will present her research finding in T cell recruitment in EAE as a poster.
The Huang lab has another spectacular year of fund-raising on behalf of the St. Baldrick's Foundation. Dr. Huang, Jay Myers, and Jennifer Tsai were part of the Rainbow Babies & Children's Hospital Team which raised a record $23,000+. Dr. Huang was among the top 10 personal fundraisers in the city of Cleveland, with more than $4700.
Congratulations to Jennifer Tsai, CWRU SOM for being named a St. Baldrick's Summer Fellow for her work in characterizing antigen presenting cell populations in the CNS of EAE mosue. The award is for a total of $5000 for one year.
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